Transcriptome of Aphanomyces euteiches: New Oomycete Putative Pathogenicity Factors and Metabolic Pathways

• DOI: 10.1371/journal.pone.0001723
• Publication Date: 2008-03-04T22:54:50Z
• Authors: Elodie Gaulin; Mohammed-Amine Ma...; Arnaud Bottin; Christophe Jacquet; Catherine Mathé; Arnaud Couloux; Patrick Wincker; Bernard Dumas
• Keywords: Oomycete; Proteome; Root rot; Phytophthora sojae

Aphanomyces euteiches is an oomycete pathogen that causes seedling blight and root rot of legumes, such as alfalfa pea. The genus phylogenically distinct from well-studied oomycetes Phytophthora sp., contains species pathogenic on plants aquatic animals. To provide the first foray into gene diversity A. euteiches, two cDNA libraries were constructed using mRNA extracted mycelium grown in artificial liquid medium or contact to plant roots. A unigene set 7,977 sequences was obtained 18,864 high-quality expressed sequenced tags (ESTs) characterized for potential functions. Comparisons with proteomes revealed major differences between content those species, leading identification biosynthetic pathways absent Phytophthora, new putative pathogenicity genes expansion families encoding extracellular proteins, notably different classes proteases. Among specific are members a family proteins putatively involved adhesion, containing up four protein domains similar fungal cellulose binding domains. Comparison fully eukaryotic pathogens, including fungi, apicomplexa trypanosomatids, allowed close orthologs these microorganisms but other so far, transporters non-ribosomal peptide synthetases, suggests presence defense mechanism against oxidative stress which initially trypanosomatids.