S100A7-Downregulation Inhibits Epidermal Growth Factor-Induced Signaling in Breast Cancer Cells and Blocks Osteoclast Formation

• DOI: 10.1371/journal.pone.0001741
• Publication Date: 2008-03-04T22:58:10Z
• Authors: Vikram Paruchuri; Anil Prasad; Kevin McHugh; Hari K. Bhat; Kornelia Polyak; Ramesh K. Ganju
• Keywords: Matrigel

S100A7 is a small calcium binding protein, which has been shown to be differentially expressed in psoriatic skin lesions, as well squamous cell tumors of the skin, lung and breast. Although its expression correlated HER+ high-grade high risk progression, molecular mechanisms these S100A7-mediated tumorigenic effects are not known. Here, we showed for first time that epidermal growth factor (EGF) induces both MCF-7 MDA-MB-468 lines. We also observed decrease EGF-directed migration shRNA-downregulated Furthermore, our signaling studies revealed EGF induced simultaneous receptor phosphorylation at Tyr1173 HER2 Tyr1248 S100A7-downregulated lines compared vector-transfected controls. In addition, reduced Src tyrosine 416 p-SHP2 542 was downregulated Further cells had angiogenesis vivo based on matrigel plug assays. Our results decreased tumor-induced osteoclastic resorption an intra-tibial bone injection model involving SCID mice. osteoclast number size vector controls, this associated with variations IL-8 vitro cultures. This novel report role EGF-induced breast cancer formation.