A Drosophila Smyd4 Homologue Is a Muscle-Specific Transcriptional Modulator Involved in Development

• DOI: 10.1371/journal.pone.0003008
• Publication Date: 2008-08-19T21:15:23Z
• Authors: Elizabeth C. Thom...; Andrew A. Travers
• Keywords: Ultrabithorax

SET and MYND domain (Smyd) proteins are involved in the transcriptional regulation of cellular proliferation development vertebrates. However, vivo functions mechanisms by which these act poorly understood.We have used biochemical genetic approaches to study role a Smyd protein Drosophila. We identified eleven Drosophila genes that encode proteins. CG14122 encodes Smyd4 homologue we named dSmyd4. dSmyd4 repressed transcription recruited class I histone deacetylases (HDACs). A region including interacted directly with approximately 150 amino acids at N-termini dHDAC1 dHDAC3. interacts selectively Ebi, component dHDAC3/SMRTER co-repressor complex. During embryogenesis was expressed throughout mesoderm, highest levels somatic musculature. Muscle-specific RNAi against resulted depletion lead severe lethality. Eclosion is final moulting stage when adult flies escape from pupal case. 80% knockdown were not able eclose, resulting late many aspects eclosion still occur normally, indicating likely be or function muscle.Repression involvement this suggests conserved between vertebrates invertebrates.