Septins Regulate Bacterial Entry into Host Cells

• DOI: 10.1371/journal.pone.0004196
• Publication Date: 2009-01-15T02:00:06Z
• Authors: Serge Mostowy; To Nam Tham; Anne Danckaert; Stéphanie Guadagnini; Stéphanie Boisson...; Javier Pizarro-Cerdá; Pascale Cossart
• Keywords: Septin

Background Septins are conserved GTPases that form filaments and required in many organisms for several processes including cytokinesis. We previously identified SEPT9 associated with phagosomes containing latex beads coated the Listeria surface protein InlB. Methodology/Principal Findings Here, we investigated septin function during entry of invasive bacteria non-phagocytic mammalian cells. found SEPT9, its interacting partners SEPT2 SEPT11, recruited as collars next to actin at site Shigella. SEPT2-depletion by siRNA decreased bacterial invasion, suggesting septins have roles particle entry. Incubating cells InlB-coated confirmed an essential role SEPT2. Moreover, impaired InlB-mediated stimulation Met-dependent signaling shown FRET. Conclusions/Significance Together these findings highlight novel SEPT2, distinguish