The Vibrio cholerae Cytolysin Promotes Chloride Secretion from Intact Human Intestinal Mucosa

• DOI: 10.1371/journal.pone.0005074
• Publication Date: 2009-03-30T23:02:20Z
• Authors: Lucantonio Debellis; Anna Diana; Diletta Arcidiacono; Romina Fiorotto; Piero Portincasa; Donato Francesco ...; Carlo Spirlì; Marina de Bernard
• Keywords: Cholera toxin; El Tor; Cholera; Virulence factor; Ussing chamber; Cytolysin

The pathogenicity of the Vibrio cholerae strains belonging to serogroup O1 and O139 is due production virulence factors such as cholera toxin (CT) toxin-coregulated pilus (TCP). remaining serogroups, which mostly lack CT TCP, are more frequently isolated from aquatic environmental sources than clinical samples; nevertheless, these have been reported cause human disease, sporadic outbreaks watery diarrhoea inflammatory enterocolitis. This evidence suggested possibility that other factor(s) might be crucial in pathogenesis cholerae-induced diarrhoea, but their nature remains unknown. VCC, hemolysin produced by virtually all strains, has proposed a possible candidate, though clear-cut demonstration attesting VCC still lacking.Electrophysiological parameters paracellular permeability stripped healthy colon tissues, obtained at subtotal colectomy, mounted Ussing chamber were studied presence or absence purified culture supernatants V. El Tor strain. Short circuit current (I(SC)) transepithelial resistance (R(T)) measured computerized voltage clamp system. exposure sigmoid specimens 1 nM resulted an increase I(SC) 20.7%, with respect basal values, while R(T) was reduced 12.3%. Moreover, abolished bilateral Cl(-) reduction.Our results demonstrate forming anion channels on apical membrane enterocytes, triggers outward transcellular flux chloride. Such ion movement, associated movement Na(+) water, responsible for caused non-toxigenic cholerae.