Genome Degradation in Brucella ovis Corresponds with Narrowing of Its Host Range and Tissue Tropism

• DOI: 10.1371/journal.pone.0005519
• Publication Date: 2009-05-12T21:32:10Z
• Authors: Renee M. Tsolis; Rekha Seshadri; Renato L. Santos; Felix J. Sangari; Juan M. García Lobo; Maarten F. de Jong; Qinghu Ren; Garry Myers; Lauren M. Brinkac; William C. Nelson; Robert T. DeBoy; Samuel Angiuoli; Hoda Khouri; George Dimitrov; Jeffrey R. Robinson; Stephanie Mulligan; Richard L. Walker; Philip E. Elzer; Karl A. Hassan; Ian T. Paulsen
• Keywords: 0301 basic medicine; 570; General Science & Technology; Science; Brucella ovis; Veterinary and Food Sciences; Microbiology; 03 medical and health sciences; Rare Diseases; Bacterial Proteins; Genetics; 2.2 Factors relating to the physical environment; Animals; Veterinary Sciences; Agricultural; Genome; Sheep; Q; Bacterial; R; Biological Sciences; Emerging Infectious Diseases; Infectious Diseases; Host-Pathogen Interactions; DNA Transposable Elements; Medicine; Infection; Gene Deletion; Genome, Bacterial; Biotechnology; Research Article

Brucella ovis is a veterinary pathogen associated with epididymitis in sheep. Despite its genetic similarity to the zoonotic pathogens B. abortus, B. melitensis and B. suis, B. ovis does not cause zoonotic disease. Genomic analysis of the type strain ATCC25840 revealed a high percentage of pseudogenes and increased numbers of transposable elements compared to the zoonotic Brucella species, suggesting that genome degradation has occurred concomitant with narrowing of the host range of B. ovis. The absence of genomic island 2, encoding functions required for lipopolysaccharide biosynthesis, as well as inactivation of genes encoding urease, nutrient uptake and utilization, and outer membrane proteins may be factors contributing to the avirulence of B. ovis for humans. A 26.5 kb region of B. ovis ATCC25840 Chromosome II was absent from all the sequenced human pathogenic Brucella genomes, but was present in all of 17 B. ovis isolates tested and in three B. ceti isolates, suggesting that this DNA region may be of use for differentiating B. ovis from other Brucella spp. This is the first genomic analysis of a non-zoonotic Brucella species. The results suggest that inactivation of genes involved in nutrient acquisition and utilization, cell envelope structure and urease may have played a role in narrowing of the tissue tropism and host range of B. ovis.