Functional and Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction

• DOI: 10.1371/journal.pone.0008443
• Publication Date: 2009-12-30T21:19:36Z
• Authors: Zongjin Li; Kitchener D. Wilson; Bryan Smith; Daniel L. Kraft; Fangjun Jia; Mei Huang; Xiaoyan Xie; Robert C. Robbins; Sanjiv S. Gambhir; Irving L. Weissman; Joseph C. Wu
• Keywords: Vasculogenesis; Embryoid body; Regenerative Medicine; Cell therapy; Therapeutic angiogenesis

Differentiation of human embryonic stem cells into endothelial (hESC-ECs) has the potential to provide an unlimited source for novel transplantation therapies ischemic diseases by supporting angiogenesis and vasculogenesis. However, differentiation efficiency conventional embryoid body (EB) method is low while 2-dimensional co-culturing with mouse fibroblasts (MEFs) require animal product, both which can limit future clinical application hESC-ECs. Moreover, fully understand beneficial effects cell therapy, investigators must be able track functional biology physiology transplanted in living subjects over time.In this study, we developed extracellular matrix (ECM) culture system increasing free from contaminating cells. We investigated transcriptional changes that occur during hESCs using whole genome microarray, compared umbilical vein (HUVECs). also showed vascular formation hESC-ECs a dorsal window model. our study first so far transplant myocardial infarction model monitor fate molecular imaging methods.Taken together, report more efficient derivation express appropriate patterns genes, form vessels vivo, improve cardiac function. These studies suggest may therapy heart disease future.