MicroRNA-145 Targets YES and STAT1 in Colon Cancer Cells

• DOI: 10.1371/journal.pone.0008836
• Publication Date: 2010-01-20T17:49:35Z
• Authors: Lea H. Gregersen; Anders B. Jacobsen; Lisa B. Frankel; Jiayu Wen; Anders Krogh; Anders H. Lund
• Keywords: gene overexpression; Science; Messenger; 610; Down-Regulation; Cell Line; 03 medical and health sciences; STAT1 protein; Cell Line, Tumor; cell growth; Humans; human; RNA, Messenger; 3' Untranslated Regions; microRNA 145; cancer cell; Proto-Oncogene Proteins c-yes; 0303 health sciences; Tumor; microRNA; human cell; Q; article; R; 3' untranslated region; unclassified drug; 3. Good health; MicroRNAs; cell death; cell proliferation; STAT1 Transcription Factor; colon cancer; protein kinase Yes; Colonic Neoplasms; gene expression; RNA; Medicine; cell cycle; microarray analysis; down regulation; Research Article

Background MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized key players in tumorigenesis. miR-145 is reported to be down-regulated several cancers, but knowledge of its targets colon cancer remains limited. Methodology/Principal Findings To investigate the role cancer, we employed a microarray based approach identify targets. Based on seed site enrichment analyses unbiased word analyses, found significant miRNA binding sites 3′-untranslated regions (UTRs) transcripts upon overexpression. Gene Ontology analysis showed an overrepresentation genes involved cell death, cellular growth proliferation, cycle, expression cancer. A number identified previously been implicated including YES, FSCN1, ADAM17, BIRC2, VANGL1 well transcription factor STAT1. Both YES STAT1 were verified direct Conclusions/Significance The study identifies validates new cancer-relevant cells hereby adds mechanistic understanding tumor-suppressive functions miR-145.