Expression of the Stress Response Oncoprotein LEDGF/p75 in Human Cancer: A Study of 21 Tumor Types

• DOI: 10.1371/journal.pone.0030132
• Publication Date: 2012-01-19T21:48:12Z
• Authors: Anamika Basu; Heather Rojas; Hiya Banerjee; Irena B. Cabrera; Kayla Y. Perez; Marino De León; Carlos A. Casiano
• Keywords: Tissue microarray

Oxidative stress-modulated signaling pathways have been implicated in carcinogenesis and therapy resistance. The lens epithelium derived growth factor p75 (LEDGF/p75) is a transcription co-activator that promotes resistance to stress-induced cell death. This protein has inflammatory autoimmune conditions, HIV-AIDS, cancer. Although LEDGF/p75 emerging as stress survival oncoprotein, there scarce information on its expression human tumors. present study was performed evaluate comprehensive panel of cancers. Transcript examined the Oncomine cancer gene microarray database TissueScan Cancer Survey Panel quantitative polymerase chain reaction (Q-PCR) array. Protein assessed by immunohistochemistry (IHC) tissue microarrays (TMAs) containing 1735 tissues representing single or replicate cores from 1220 individual cases (985 tumor 235 normal tissues). A total 21 major types were analyzed. Analysis transcript datasets revealed significant upregulation (tumor vs. normal) 15 out 17 types. Q-PCR array significantly elevated prostate, colon, thyroid, breast IHC analysis TMAs increased levels liver uterine tumors, relative corresponding tissues. Elevated observed several These results further establish cancer-related protein, provide rationale for ongoing studies aimed at understanding clinical significance specific