Differential Macrophage Activation Alters the Expression Profile of NTPDase and Ecto-5′-Nucleotidase

• DOI: 10.1371/journal.pone.0031205
• Publication Date: 2012-02-13T22:43:33Z
• Authors: Rafael Fernandes ...; Elizandra Braganhol; Letícia Scussel B...; Luís Felipe Ingra...; Alfeu Zanotto Filho; José Cláudio Fons...; Fernanda Bueno Mo...; Jean Sévigny; Maria Rosa Chitol...; Angela Terezinha ...; Ana Maria Oliveir...
• Keywords: Apyrase; 5'-nucleotidase; Nucleotidase; Macrophage-activating factor; Adenosine triphosphate

Macrophages are key elements in the inflammatory process, whereas depending on micro-environmental stimulation they exhibit a pro-inflammatory (classical/M1) or an anti-inflammatory/reparatory (alternative/M2) phenotype. Extracellular ATP can act as danger signal adenosine generally serves negative feedback mechanism to limit inflammation. The local increase nucleotides communication is controlled by ectonucleotidases, such members of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family and ecto-5′-nucleotidase/CD73 (ecto-5′-NT). In present work we evaluated presence these enzymes resident mice M1 (macrophages stimulated with LPS), M2 IL-4) macrophages. were collected lavage (6–8 weeks) peritoneal cavity treated for 24 h IL-4 (10 ng/mL) LPS ng/mL). Nitrite concentrations measured using Greiss reaction. Supernatants harvested determine cytokines ATPase, ADPase AMPase activities determined malachite green method HPLC analysis. expression selected surface proteins was flow cytometry. results reveal that macrophages presented decreased AMP hydrolysis agreement decrease NTPDase1, -3 ecto-5′-nucleotidase compared M2. contrast, showed higher increased Therefore, phenotype lead accumulation while may rapidly convert adenosine. also P1 P2 purinoreceptors same mRNA profile both phenotypes. addition, macrophages, which have ATPase activity, less sensitive cell death. conclusion, changes ectoenzyme might allow adjust outcome extracellular purinergic cascade order fine-tune their functions during set.