Differentiation and Recruitment of Th9 Cells Stimulated by Pleural Mesothelial Cells in Human Mycobacterium tuberculosis Infection

• DOI: 10.1371/journal.pone.0031710
• Publication Date: 2012-02-20T17:13:25Z
• Authors: Zhi-Jian Ye; Ming-Li Yuan; Qiong Zhou; Rong-Hui Du; Wei-Bing Yang; Xian-Zhi Xiong; Jian-Chu Zhang; Cong Wu; Shou-Ming Qin; Huan-Zhong Shi
• Keywords: Adult; 0301 basic medicine; Science; Apoptosis; Lymphocyte Activation; Interferon-gamma; 03 medical and health sciences; Cell Movement; Humans; Antigen Presentation; Antigens, Bacterial; 0303 health sciences; Chemokine CCL20; Ionomycin; Q; R; Interleukin-9; Cell Differentiation; Epithelial Cells; Mycobacterium tuberculosis; Middle Aged; 3. Good health; Pleural Effusion; Phenotype; Medicine; Pleura; Receptors, Chemokine; Interleukin-4; Research Article

Newly discovered IL-9-producing CD4(+) helper T cells (Th9 cells) have been reported to contribute tissue inflammation and immune responses, however, differentiation regulation of Th9 in tuberculosis remain unknown. In the present study, our data showed that increased with phenotype effector memory were found be tuberculous pleural effusion as compared blood. TGF-β was essential for cell from naïve stimulated PMA ionomycin vitro 5 h, addition IL-1β, IL-4 or IL-6 further augmented differentiation. Tuberculous supernatants cultured mesothelial chemotactic cells, this activity partly blocked by anti-CCL20 antibody. IL-9 promoted repairing inhibited IFN-γ-induced apoptosis. Moreover, presenting antigen. Collectively, these provide new information concerning particular collaborative between human M. infection. particular, able function antigen-presenting stimulate