Milk Fermented by Propionibacterium freudenreichii Induces Apoptosis of HGT-1 Human Gastric Cancer Cells

• DOI: 10.1371/journal.pone.0031892
• Publication Date: 2012-03-19T21:02:09Z
• Authors: Fabien J. Cousin; Sandrine Jouan-La...; Marie-Thérèse Dim...; Laurent Corcos; Gwénaël Jan
• Keywords: Propionibacterium freudenreichii; Propionibacterium

Background Gastric cancer is one of the most common cancers in world. The "economically developed countries" life style, including diet, constitutes a risk factor favoring this cancer. Diet modulation may lower digestive incidence. Among promising food components, dairy propionibacteria were shown to trigger apoptosis human colon cells, via release short-chain fatty acids acetate and propionate. Methodology/Principal Findings A fermented milk, exclusively by P. freudenreichii, was recently designed. In work, pro-apoptotic potential new milk demonstrated on HGT-1 gastric cells. Fermented supernatant induced typical features chromatin condensation, formation apoptotic bodies, DNA laddering, cell cycle arrest emergence subG1 population, phosphatidylserine exposure at plasma membrane outer leaflet, reactive oxygen species accumulation, mitochondrial transmembrane disruption, caspase activation cytochrome c release. Remarkably, containing freudenreichii enhanced cytotoxicity camptothecin, drug used chemotherapy. Conclusions/Significance Such probiotic thus be useful as part preventive diet designed prevent and/or supplement potentiate therapeutic treatments.