The Respiratory Syncytial Virus G Protein Conserved Domain Induces a Persistent and Protective Antibody Response in Rodents

• DOI: 10.1371/journal.pone.0034331
• Publication Date: 2012-03-29T21:19:34Z
• Authors: Thien N. Nguyen; Ultan F. Power; Alain Robert; Jean-François Haeuw; Katia Helffer; Amadeo Perez; Miguel-Angel Asin; Nathalie Corvaia; Christine Libon
• Keywords: Diphtheria Toxin

Respiratory syncytial virus (RSV) is an important cause of severe upper and lower respiratory disease in infants the elderly. There are 2 main RSV subtypes A B. recombinant vaccine was designed based on central domain RSV-A attachment G protein which we had previously named G2Na (aa130-230). Here evaluated immunogenicity, persistence antibody (Ab) response protective efficacy induced rodents by: (i) fused to DT (Diphtheria toxin) fragments cotton rats. fusion did not potentiate neutralizing Ab responses against or cross-reactivity RSV-B. (ii) G2Nb (aa130-230 RSV-B protein) either to, admixed with G2Na. induce RSV-B-reactive responses. (iii) at low doses. Two injections 3 µg Alum were sufficient immune mouse lungs, preventing greatly reducing infections. In rats, G2Na-induced RSV-reactive immunity challenge that persisted for least 24 weeks. (iv) injecting primed mice a single dose G2Na/Alum G2Na/PLGA [poly(D,L-lactide-co-glycolide]. Despite presence pre-existing RSV-specific Abs, these formulations effectively boosted anti-RSV titres increased 21 Affinity maturation Abs from day 28 148. These data indicate has potential as component formulation.