Increased Percentages of T Helper Cells Producing IL-17 and Monocytes Expressing Markers of Alternative Activation in Patients with Sepsis

• DOI: 10.1371/journal.pone.0037393
• Publication Date: 2012-05-31T22:27:24Z
• Authors: Milena Karina Col...; Michelle Carolina...; Otelo Rigato; Flavia Ribeiro Ma...; Eliezer Silva; Reinaldo Salomao
• Keywords: CD163; Monocyte

Background A shift from Th1 to Th2 as well an increase in Treg CD4+T cell subsets has been reported septic patients (SP). Furthermore, these display modulation of monocyte function, with reduced production pro-inflammatory cytokines upon LPS stimulus, which resembles the phenotype alternatively activated macrophages. In this study, we evaluated percentages T cells differentiated into Th1, Th17 and subsets, percentage monocytes expressing markers monocytes/macrophages (AAM) SP. Methodology/Principal Findings Peripheral blood mononuclear (PBMC) were obtained 32 healthy volunteers (HV) SP at admission (D0, n = 67) after 7 days therapy (D7, 33). (CD3+CD8−) lymphocytes identified by intracellular detection IFN-γ IL-17, respectively, spontaneously PMA/Io stimulation, Foxp3+CD127- expression. Monocytes for CD206 CD163 Absolute numbers measured whole samples flow cytometry. The Mann-Whitney or Wilcoxon test was applied, appropriate. lower than HV whereas higher. patients' follow-up samples, a higher observed on D7 compared D0 samples. remained unchanged. Septic showed markedly increased proportion CD206. Conclusions/Significance Upon vitro helper producing IL-17 admission, lower, pattern that reversed during follow-up. expression indicates may acquire AAM sepsis.