Fibroin and Sericin from Bombyx mori Silk Stimulate Cell Migration through Upregulation and Phosphorylation of c-Jun

• DOI: 10.1371/journal.pone.0042271
• Publication Date: 2012-07-31T21:22:19Z
• Authors: Celia Martínez-Mora; Anna Mrowiec; Eva María García-...; Antonia Alcaraz; José Luis Cenis; Francisco José Ni...
• Keywords: Fibroin; Sericin; HaCaT

Wound healing is a biological process directed to the restoration of tissue that has suffered an injury. An important phase wound generation basal epithelium able wholly replace epidermis wound. A broad range products derived from fibroin and sericin Bombyx mori silk are used stimulate healing. However, so far molecular mechanism underlying this phenomenon not been elucidated. The aim work was determine basis properties proteins using cell model. For purpose, we assayed in scratch assay MDA-MB-231 Mv1Lu cells. Both stimulated migration. Furthermore, treatment with involved key factors such as upregulation c-Jun protein phosphorylation. Moreover, phosphorylation ERK 1/2 JNK kinases. All these experiments were done presence specific inhibitors for some signalling pathways referred above. obtained results revealed MEK, PI3K cells Inhibition three kinases prevented by or sericin. Fibroin tested human keratinocyte line, HaCaT, similar results. Altogether, our showed initiate migration activating ending activation.