A RAD Tag Derived Marker Based Eggplant Linkage Map and the Location of QTLs Determining Anthocyanin Pigmentation

• DOI: 10.1371/journal.pone.0043740
• Publication Date: 2012-08-17T17:29:09Z
• Authors: Lorenzo Barchi; Sergio Lanteri; Ezio Portis; Giampiero Valè; Andrea Volante; Laura Pulcini; Tommaso Ciriaci; Nazareno Acciarri; Valeria Barbierato; Laura Toppino; Giuseppe Leonardo...
• Keywords: Candidate gene; Genetic linkage; Family-based QTL mapping; Marker-Assisted Selection; Synteny; Association mapping

Both inter- and intra-specific maps have been developed in eggplant (Solanum melongena L.). The former benefit from an enhanced frequency of marker polymorphism, but their relevance to marker-assisted crop breeding is limited. Combining the restriction-site associated DNA strategy with high throughput sequencing has facilitated discovery a large number functional single nucleotide polymorphism (SNP) markers discriminating between two mapping population parental lines '305E40' '67/3'. A set 347 de novo SNPs, together 84 anchoring markers, were applied F2 bred cross x '67/3' construct linkage map. In all, 415 431 assembled into twelve major one minor group, spanning 1,390 cM, inclusion established allowed each group be assigned 12 chromosomes. map was then used discover genetic basis seven traits anthocyanin content. Each proved controlled by six quantitative trait loci (QTL), which at least QTL. Exploitation syntenic relationships tomato genomes identification potential candidate genes for QTLs related accumulation. should utility elucidating other phenotypic eggplant.