Molecular Parentage Analysis Is Essential in Breeding Asian Seabass

Male Population Density 570 0303 health sciences Asia Science Q R Genetic Variation Breeding 630 03 medical and health sciences Gene Frequency Medicine Animals Bass Female 14. Life underwater Alleles Crosses, Genetic Research Article Microsatellite Repeats
DOI: 10.1371/journal.pone.0051142 Publication Date: 2012-12-06T00:26:22Z
ABSTRACT
In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males ×10 females) of Asian seabass reconstruct monitor the change variations. all crosses, over 96.8% could be assigned their parents, indicating high power for parentage assignment. This study revealed several interesting results: (1). contribution parents was significantly uneven, some dominant breeding fishes (i.e. brooders) were found; (2). two mass where brooders carefully checked reproductive status, a majority (≥90%) contributed offspring, whereas another randomly picked without checking only few (40.0–45.0%) produced offspring; (3). Females had more problems successful spawning compared males; (4). there substantial loss allelic (24.1–34.3%) gene (20.5–25.7%) diversities while other (96.8–97.0%) (94.8–97.1%) maintained. These observations suggest that routine molecular analysis required maintain both diversity seabass.
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