Activated ERM Protein Plays a Critical Role in Drug Resistance of MOLT4 Cells Induced by CCL25

• DOI: 10.1371/journal.pone.0052384
• Publication Date: 2013-01-10T00:17:31Z
• Authors: Li Zhang; Ruijing Xiao; Jie Xiong; Jun Leng; Altaf Ehtisham; Yi Hu; Qianshan Ding; Hui Xu; Shengwu Liu; Jin Wang; Dean G. Tang; Qiuping Zhang
• Keywords: CCL25

We have previously demonstrated that the CCR9/CCL25 signaling pathway plays an important role in drug resistance human acute T-lymphocytic leukemia (T-ALL) by inducing activation of ERM protein with polarized distribution T-ALL cell line MOLT4. However, mechanism action activated MOLT4 cells induced CCL25 remains uncharacterized. Here we investigated CCR9/CCL25-initiated CCR9-high-expressing cells. Our results showed 1) function P-gp was increased after treatment CCL25; 2) colocalized and co-immunoprecipitated p-ERM F-actin treated cells; 3) ERM-shRNA conferred sensitivity coincident release interactions CCL25. These data suggest it is pivotal associate cytoskeleton through multidrug Strategies aimed at inhibiting P-gp-F-actin association may be helpful increasing efficiency therapies T-ALL.