CBP and p300 Histone Acetyltransferases Contribute to Homologous Recombination by Transcriptionally Activating the BRCA1 and RAD51 Genes

• DOI: 10.1371/journal.pone.0052810
• Publication Date: 2012-12-20T22:42:20Z
• Authors: Hideaki Ogiwara; Takashi Kohno
• Keywords: Chromatin immunoprecipitation

Histone acetylation at DNA double-strand break (DSB) sites by CBP and p300 histone acetyltransferases (HATs) is critical for the recruitment of DSB repair proteins to chromatin. Here, we show that HATs also function in transcriptionally activating BRCA1 RAD51 genes, which are involved homologous recombination (HR), a major system. siRNA-mediated depletion impaired HR activity downregulated protein mRNA levels. Chromatin immunoprecipitation assays showed bind promoter regions and/or reduces H3 H4 inhibits binding transcription factor E2F1 these promoters. Depletion damage-induced phosphorylation chromatin single-strand DNA-binding RPA following BRCA1-mediated end resection. Consistent with this, subsequent CHK1 activation G2/M damage checkpoint were impaired. These results indicate play multiple roles cellular response DSBs.