Nanoceria: A Rare-Earth Nanoparticle as a Novel Anti-Angiogenic Therapeutic Agent in Ovarian Cancer

• DOI: 10.1371/journal.pone.0054578
• Publication Date: 2013-01-31T22:03:23Z
• Authors: Shailendra Giri; Ajay Karakoti; Rondell P. Graham; Jacie L. Maguire; Christopher M. Re...; Sudipta Seal; Ramandeep Rattan; Viji Shridhar
• Keywords: 0301 basic medicine; Science; BEVACIZUMAB; Mice, Nude; Angiogenesis Inhibitors; CERIUM OXIDE NANOPARTICLES; TUMOR ANGIOGENESIS; ACTIVATION; Mice; 03 medical and health sciences; Cell Movement; KRABBE-DISEASE; Cell Line, Tumor; Animals; Humans; Neoplasm Metastasis; IN-VIVO; Cell Proliferation; Ovarian Neoplasms; Neovascularization, Pathologic; Vascular Endothelial Growth Factors; Q; R; Endothelial Cells; Cerium; Tumor Burden; 3. Good health; Multidisciplinary Sciences; Disease Models, Animal; ENDOTHELIAL GROWTH-FACTOR; CELLS; METASTASIS; PHASE-II; Medicine; Nanoparticles; Female; Reactive Oxygen Species; Research Article; Signal Transduction

Ovarian cancer (OvCa) is the fifth most common cause of death from all cancers among women in United Sates and leading gynecological malignancies. While OvCa patients initially respond to surgical debulking chemotherapy, 75% later succumb disease. Thus, there an urgent need test novel therapeutic agents counteract high mortality rate associated with OvCa. In this context, we have developed engineered Nanoceria (NCe), nanoparticles cerium oxide, possessing anti-oxidant properties, be used as a agent We show for first time that NCe significantly inhibited production reactive oxygen species (ROS) A2780 cells, attenuated growth factor (SDF1, HB-EGF, VEGF165 HGF) mediated cell migration invasion SKOV3 without affecting proliferation. treatment also induced proliferation, capillary tube formation, activation VEGFR2 MMP2 human umbilical vascular endothelial cells (HUVEC). (0.1 mg/kg body weigh) ovarian injected intra-peritoneally nude mice showed significant reduction (p<0.002) tumor accompanied by decreased proliferation evident reduced size Ki67 staining. Accumulation was found tumors isolated treated group using transmission electron microscopy (TEM) inductively coupled plasma mass spectroscopy (ICP-MS). Reduction attenuation angiogenesis, observed CD31 staining specific apoptosis cells. Collectively, these results indicate oxide based nanoparticle can potentially anti-angiogenic cancer.