MicroRNA-195 Inhibits the Proliferation of Human Glioma Cells by Directly Targeting Cyclin D1 and Cyclin E1

• DOI: 10.1371/journal.pone.0054932
• Publication Date: 2013-01-29T00:42:38Z
• Authors: Wang Hui; Lu Yuntao; Luo Lun; Li WenSheng; Liang ChaoFeng; He HaiYong; Ba Yueyang
• Keywords: Cyclin E1

Glioma proliferation is a multistep process during which sequence of genetic and epigenetic alterations randomly occur to affect the genes controlling cell proliferation, death stability. microRNAs are emerging as important modulators multiple target genes, leading abnormal cellular signaling involving in cancers.In present study, we found that expression miR-195 was markedly downregulated glioma lines human primary tissues, compared normal astrocytes matched non-tumor associated tissues. Upregulation dramatically reduced cells. Flow cytometry analysis showed ectopic significantly decreased percentage S phase cells increased G1/G0 Overexpression anchorage-independent growth ability Furthermore, overexpression levels phosphorylated retinoblastoma (pRb) proliferating nuclear antigen (PCNA) Conversely, inhibition promoted cells, enhanced ability, upregulated phosphorylation pRb PCNA Moreover, show inhibited by downregulating cyclin D1 E1, via directly targeting 3′-untranslated regions (3′-UTR) E1 mRNA. Taken together, our results suggest plays an role inhibit novel mechanism for direct miRNA-mediated suppression glioma.