A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

• DOI: 10.1371/journal.pone.0061801
• Publication Date: 2013-04-16T20:59:14Z
• Authors: Xiao-jun Yang; Wei Cui; Ai Gu; Chuan Xu; Shi-cang Yu; Ting-ting Li; You-hong Cui; Xia Zhang; Xiu-wu Bian
• Keywords: Xenotransplantation; U87; Danio

Invasion and metastasis of solid tumors are the major causes death in cancer patients. Cancer stem cells (CSCs) constitute a small fraction tumor cell population, but play critical role invasion metastasis. The xenograft immunodeficient mice is one commonly used vivo models to study cells. However, this model time-consuming labor intensive. Zebrafish (Danio rerio) their transparent embryos emerging as promising system for studies invasion. In study, we established by using zebrafish embryo xenografted with human glioblastoma line U87 its derived (CSCs). We found that CSCs-enriched from spreaded via vessels within such displayed an extremely high level invasiveness which was associated up-regulated MMP-9 CSCs. glioma CSCs (GSCs) markedly inhibited inhibitor. Thus, our considered cost-effective approach tostudies mechanisms underlying suitable high-throughput screening novel anti-tumor invasion/metastasis agents.