Peripheral Monocyte Functions and Activation in Patients with Quiescent Crohn’s Disease

• DOI: 10.1371/journal.pone.0062761
• Publication Date: 2013-04-26T21:04:39Z
• Authors: David Schwarzmaier; Dirk Foell; Toni Weinhage; Georg Varga; Jan Däbritz
• Keywords: Monocyte; CD16

Recent developments suggest a causal link between inflammation and impaired bacterial clearance in Crohn's disease (CD) due to alterations of intestinal macrophages. Studies that excessive is the consequence an underlying immunodeficiency rather than primary cause CD pathogenesis. We characterized phenotypic functional features peripheral blood monocytes patients with quiescent (n = 18) healthy controls 19) by analyses cell surface molecule expression, adherence, migration, chemotaxis, phagocytosis, oxidative burst, cytokine expression secretion or without lipopolysaccharide (LPS) priming. Peripheral inactive showed normal molecules (CD14, CD16, CD116), adherence plastic surfaces, spontaneous chemotaxis towards LTB4, phagocytosis E. coli, production reactive oxygen species. Interestingly, secreted higher levels IL1β (p<.05). Upon LPS priming we found decreased release IL10 (p<.05) CCL2 (p<.001) CCL5 The TNFα, IFNγ, IL4, IL6, IL8, IL13, IL17, CXCL9, CXCL10 were not altered compared controls. Based on our studies, from clinical remission Our results highlight defective innate immune mechanisms seems play role (inflamed) mucosa blood.