Therapeutic Vaccines against Human and Rat Renin in Spontaneously Hypertensive Rats

• DOI: 10.1371/journal.pone.0066420
• Publication Date: 2013-06-25T21:13:48Z
• Authors: Zhihua Qiu; Xiao Chen; Yanzhao Zhou; Jibin Lin; Dan Ding; Shijun Yang; Fen Chen; Min Wang; Feng Zhu; Xian Yu; Zihua Zhou; Yuhua Liao
• Keywords: Keyhole limpet hemocyanin; Plasma renin activity; Antibody titer; Antigenicity

Vaccination provides a promising approach for treatment of hypertension and improvement in compliance. As the initiation factor renin-angiotensin system, renin plays critical role hypertension. In this study, we selected six peptides (rR32, rR72, rR215, hR32, hR72, hR215) belonging to potential epitopes rat human renin. The main criteria were as follows: (1) include one catalytic sites or flap sequence; (2) low/no-similarity when matched with host proteome; (3) ideal antigenicity hydrophilicity. coupled keyhole limpet hemocyanin injected into SpragueDawley (SD) rats, spontaneously hypertensive rats (SHRs) Wistar-Kyoto rats. antisera titers binding capacity detected. effects anti-peptides antibodies on plasma activity (PRA) blood pressure also determined. All elicited strong antibody responses. ranged from 1:32,000 1:80,000 SD day 63. could bind vitro. Compared control antibody, against rR32, rR72 hR72 inhibited PRA level by up about 50%. Complete cross-reactivity anti-rR32 anti-hR32 was confirmed. rR32 hR32 vaccines significantly decreased systolic (SBP) SHRs 15mmHg (175±2 vesus 190±3 mmHg, P = 0.035; 180±2 195±3 0.039), while no obvious effect Additionally, significant immune-mediated damage detected vaccinated animals. conclusion, antigenic peptide vaccine mimicking 32Asp site may constitute novel tool development vaccine.