Gene Expression Profiling of Immune-Competent Human Cells Exposed to Engineered Zinc Oxide or Titanium Dioxide Nanoparticles

• DOI: 10.1371/journal.pone.0068415
• Publication Date: 2013-07-22T21:05:34Z
• Authors: Soile Tuomela; Reija Autio; Tina Buerki-Thurn...; Osman Arslan; Andrea Kunzmann; Britta Andersson-...; Peter Wick; Sanjay Mathur; Annika Scheynius; Harald F. Krug; Bengt Fadeel; Riitta Lahesmaa
• Keywords: Jurkat cells; Nanotoxicology

A comprehensive in vitro assessment of two commercial metal oxide nanoparticles, TiO2 and ZnO, was performed using human monocyte-derived macrophages (HMDM), dendritic cells (MDDC), Jurkat T cell leukemia-derived line. nanoparticles were found to be non-toxic whereas ZnO caused dose-dependent death. Subsequently, global gene expression profiling identify transcriptional response underlying the cytotoxicity by nanoparticles. Analysis done with doses 1 µg/ml 10 after 6 24 h exposure. Interestingly, 2703 genes significantly differentially expressed HMDM upon exposure while MDDCs only 12 affected. In cells, 980 expressed. It is noteworthy that metallothioneins upregulated all three types a notable proportion regulated type-specific manner. Gene ontology analysis revealed top biological processes disturbed regulating death growth. addition, controlling immune system development Using panel modified we obtained an additional support cellular largely dependent on particle dissolution show ligand used modify modulates Zn2+ leaching. Overall, study provides extensive resource markers for mediating nanoparticle-induced toxicity further mechanistic studies, demonstrates value assessing nanoparticle responses through combined transcriptomics bioinformatics approach.