The Synergistic Immunoregulatory Effects of Culture-Expanded Mesenchymal Stromal Cells and CD4+25+Foxp3+ Regulatory T Cells on Skin Allograft Rejection

• DOI: 10.1371/journal.pone.0070968
• Publication Date: 2013-08-05T21:30:51Z
• Authors: Jung Ho Lee; Eun-Joo Jeon; Nayoun Kim; Young-Sun Nam; Keon-Il Im; Jung-Yeon Lim; Eun-Jung Kim; Mi-La Cho; Ki Taik Han; Seok-Goo Cho
• Keywords: Cell therapy; Regulatory T cell; Splenocyte

Mesenchymal stromal cells (MSCs) are seen as an ideal source of to induce graft acceptance; however, some reports have shown that MSCs can be immunogenic rather than immunosuppressive. We speculate the immunomodulatory effects regulatory T (Tregs) aid maintenance immunoregulatory functions MSCs, and a combinatorial approach cell therapy synergistic on allograft rejection. After preconditioning with Fludarabine, followed by total body irradiation anti-asialo-GM-1(ASGM-1), tail skin grafts from C57BL/6 (H-2k(b)) mice were grafted onto lateral thoracic wall BALB/c (H-2k(d)) mice. Group A (control group, n = 9) did not receive any further treatment after preconditioning, whereas groups B C (n received or Tregs, respectively, days -1, +6 +13 relative transplantation. D 10) Tregs +13. Cell suspensions obtained spleens five randomly chosen each group day +7, evaluated flow cytometry real-time PCR. Our results show survival was significantly longer in compared control (group A). Flow cytometric analysis PCR for splenocytes revealed Th2 subpopulation increased B. Also, expression Foxp3 STAT 5 conventional (B C). Taken together, these data suggest combined has effect function vivo, might provide novel strategy improving