Profiling of MicroRNA in Human and Mouse ES and iPS Cells Reveals Overlapping but Distinct MicroRNA Expression Patterns

• DOI: 10.1371/journal.pone.0073532
• Publication Date: 2013-09-24T01:47:57Z
• Authors: Siti Razila Abdul...; Kazuko Ueno; Naoya Takayama; Naoki Nariai; Masao Nagasaki; Rika Saito; Hideto Koso; Chen-Yi Lai; Miyako Murakami; Koichiro Tsuji; Tatsuo Michiue; Hiromitsu Nakauchi; Makoto Otsu; Sumiko Watanabe
• Keywords: Embryoid body; Cell type

Using quantitative PCR-based miRNA arrays, we comprehensively analyzed the expression profiles of miRNAs in human and mouse embryonic stem (ES), induced pluripotent (iPS), somatic cells. Immature cells were purified using SSEA-1 or SSEA-4 used for profiling. Hierarchical clustering consensus by nonnegative matrix factorization showed two major clusters, ES/iPS other cell groups, as previously reported. Principal components analysis (PCA) to identify that segregate these groups identified miR-187, 299-3p, 499-5p, 628-5p, 888 new specifically characterize Detailed direct comparisons levels ES iPS several included chromosome 19 cluster more strongly expressed than Similar was conducted with cells, had not been reported be suggested cell-specific PCA. Comparison average humans mice quite similar patterns human/mouse miRNAs. However, mouse- human-specific are ranked high expressers. Time course tracing during embryoid body formation revealed drastic different changes their levels. In summary, our profiling encompassing gave various perspectives understanding core regulatory networks regulating characteristics.