Transcriptome and Expression Profile Analysis of Highly Resistant and Susceptible Banana Roots Challenged with Fusarium oxysporum f. sp. cubense Tropical Race 4

• DOI: 10.1371/journal.pone.0073945
• Publication Date: 2013-09-24T01:46:57Z
• Authors: Ting-Ting Bai; Wan-Bin Xie; Ping-Ping Zhou; Zi-Lin Wu; Wen-Chao Xiao; Ling Zhou; Jie Sun; Xiao-Lei Ruan; Hua-Ping Li
• Keywords: Senescence

Banana wilt disease, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense 4 (Foc4), is regarded as one of most devastating diseases worldwide. Cavendish cultivar 'Yueyoukang 1' was shown to have significantly lower disease severity and incidence compared with susceptible 'Brazilian' in greenhouse field trials. De novo sequencing technology previously performed investigate defense mechanism middle resistant 'Nongke No banana, but not highly 1'. To gain more insights into resistance banana against Foc4, Illumina Solexa utilized perform transcriptome characterize gene expression profile changes both two cultivars at days 0.5, 1, 3, 5 10 after infection Foc4. The results showed that massive transcriptional reprogramming occurs due Foc4 treatment than 'Brazilian', especially first three time points, which suggested had much faster response 'Brazilian'. Expression patterns genes involved 'Plant-pathogen interaction' 'Plant hormone signal transduction' pathways were analyzed between cultivars. Defense associated CEBiP, BAK1, NB-LRR proteins, PR transcription factor cell wall lignification expressed stronger indicating these play important roles infection. However, related hypersensitive reaction (HR) senescence up-regulated down-regulated 1', HR may contribute In addition, found differ from banana. These explain provide understanding compatible incompatible interactions