Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital

• DOI: 10.1371/journal.pone.0081604
• Publication Date: 2014-01-17T21:36:32Z
• Authors: Lázaro Molina; Zulema Udaondo; Estrella Duque; Matilde Fernández; Carlos Molina-San...; Amalia Roca; Mario Porcel; Jesús de la Torre; Ana Segura; Patrick Plesiat; Katy Jeannot; Juan-Luis Ramos
• Keywords: 0301 basic medicine; Genomic Islands; Antibiotic resistance; Science; Molecular Sequence Data; Microbial Sensitivity Tests; 03 medical and health sciences; Bacterial Proteins; Humans; Amino Acid Sequence; Pseudomonas putida; Q; R; Drug Resistance, Microbial; Sequence Analysis, DNA; Chromosomes, Bacterial; Strain isolated; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; Hospitals; Anti-Bacterial Agents; 3. Good health; Medicine; Genome, Bacterial; Research Article; Plasmids

Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from in-patients in different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority of the antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics, macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. To shed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genome of this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are both chromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibiotic and biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens. The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts.