Characterization of a Small Auxin-Up RNA (SAUR)-Like Gene Involved in Arabidopsis thaliana Development

• DOI: 10.1371/journal.pone.0082596
• Publication Date: 2013-11-27T23:46:45Z
• Authors: Marios Nektarios ...; Agnieszka Karolin...; Bram Van Loock; Kumud Saini; Susanna Cirera; Jean-Pierre Verbelen; Kris Vissenberg
• Keywords: Lateral root; Pericycle; Primordium

The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature cell elongation and its arrest. From micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), precursor ethylene, Small auxin-up RNA (SAUR)-like gene was found be up regulated. As it appeared 76th in family, named SAUR76. Root leaf growth overexpression lines ectopically expressing SAUR76 indicated possible involvement division process. Using promoter::GUS GFP strong expression seen endodermal pericycle cells at end zone during several stages lateral primordia development. ACC IAA/NAA able induce regulation changed towards cortical even epidermal beginning zone. Confirmation this delivered using qPCR, which also quickly returned normal levels when inducing IAA-stimulus removed, behaviour other SAUR genes. Furthermore, confocal analysis protein-GFP fusions localized protein nucleus, cytoplasm plasma membrane. quantified mutants ethylene auxin-related pathways, led conclusion mainly regulated by increase auxin results from addition ACC, rather than itself.