PTK 7 Is a Transforming Gene and Prognostic Marker for Breast Cancer and Nodal Metastasis Involvement

• DOI: 10.1371/journal.pone.0084472
• Publication Date: 2014-01-07T21:26:26Z
• Authors: Silvia Gärtner; Angela Gunesch; Tatiana Knyazeva; Petra Wolf; Bernhard Högel; Wolfgang Eiermann; Axel Ullrich; Pjotr Knyazev; Beyhan Ataseven
• Keywords: Triple-negative breast cancer

Protein Tyrosin Kinase 7 (PTK7) is upregulated in several human cancers; however, its clinical implication breast cancer (BC) and lymph node (LN) still unclear. In order to investigate the function of PTK7 mediating BC cell motility invasivity, expression lines was determined. signaling highly invasive cells inhibited by a dominant-negative mutant, an antibody against extracellular domain PTK7, siRNA knockdown PTK7. This resulted decreased invasivity cells. We further examined LN tissue 128 patients RT-PCR correlation with related genes like HER2, HER3, PAI1, MMP1, K19, CD44. Expression profiling primary tumors showed association ER/PR/HER2-negative (TNBC-triple negative BC) cancer. Oncomine data analysis confirmed this observation classified cluster associated agressive behavior BC. Furthermore significantly different respect tumor size (ANOVA, p = 0.033) nodal involvement 0.007) LN. metastatic shorter DFS (Cox Regression, 0.041). Our observations transforming potential as well expressed TNBC lines. It represents novel prognostic marker for has therapeutic significance.