Expression of miR-29c, miR-93, and miR-429 as Potential Biomarkers for Detection of Early Stage Non-Small Lung Cancer

• DOI: 10.1371/journal.pone.0087780
• Publication Date: 2014-02-11T16:48:19Z
• Authors: Wangyu Zhu; Jianying He; Dongdong Chen; Bingjie Zhang; Liyun Xu; Haijie Ma; XiaoGuang Liu; YongKui Zhang; Hanbo Le
• Keywords: TaqMan; Carcinoembryonic antigen

Background Altered expression of miRNA contributes to human carcinogenesis. This study was designed detect aberrant expressions as a potential biomarker for early detection and prognosis prediction non-small cell lung cancer (NSCLC). Methods array used profile differentially expressed miRNAs Taqman-based quantitative RT-PCR assays were analyze levels miR-29c, miR-93, miR-429 in NSCLC tissue samples, corresponding normal serum samples from 70 patients well 48 healthy controls. Results Levels miR-29c miR-93 upregulated tissues, while also upregulated, but decreased NSCLC. Moreover, the tissues associated with those samples. Receiver operating characteristic (ROC) curve analysis showed that at optimal cut-off point, areas under ROC 0.723 0.727, respectively, which are higher than carcinoma embryonic antigen (0.534) diagnosis stage I In addition, poor overall survival patients. Both univariate multivariate analyses level an independent prognostic predictor Conclusions The results current suggest should be further evaluated novel, non-invasive