The Kiwifruit Emerging Pathogen Pseudomonas syringae pv. actinidiae Does Not Produce AHLs but Possesses Three LuxR Solos

• DOI: 10.1371/journal.pone.0087862
• Publication Date: 2014-01-31T23:00:04Z
• Authors: Hitendra Kumar Patel; Patrizia Ferrante; Sonia Covaceuszach; Doriano Lamba; Marco Scortichini; Vittorio Venturi
• Keywords: Pseudomonas syringae; Homoserine

Pseudomonas syringae pv. actinidiae (Psa) is an emerging phytopathogen causing bacterial canker disease in kiwifruit plants worldwide. Quorum sensing (QS) gene regulation plays important roles many different plant pathogens. In this study we analyzed the presence and possible role of N-acyl homoserine lactone (AHL) quorum Psa. It was established that Psa does not produce AHLs a typical complete LuxI/R QS system absent strains. however possesses three putative luxR solos designated here as PsaR1, PsaR2 PsaR3. belongs to sub-family LuxR present associated bacteria (PAB) binds responds yet unknown signal molecules. PsaR1 PsaR3 are highly similar LuxRs which bind part canonical AHL systems. Mutation all showed reduction planta survival also additive effect if more than one solo inactivated double mutants. Gene promoter analysis revealed auto-regulated investigated their several phenotypes.