Hepatitis B Virus Induces Cell Proliferation via HBx-Induced microRNA-21 in Hepatocellular Carcinoma by Targeting Programmed Cell Death Protein4 (PDCD4) and Phosphatase and Tensin Homologue (PTEN)

• DOI: 10.1371/journal.pone.0091745
• Publication Date: 2014-03-14T20:32:31Z
• Authors: Preeti Damania; Bijoya Sen; Sadaf Bashir Dar; Satendra Kumar; Anupama Kumari; Ekta Gupta; Shiv Kumar Sarin; Senthil Kumar Ven...
• Keywords: HBx; Tensin

Hepatitis B viral infection-induced hepatocellular carcinoma is one of the major problems in developing countries. One HBV proteins, HBx, modulates host cell machinery via several mechanisms. In this study we hypothesized that enhances proliferation HBx-induced microRNA-21 carcinoma. HBx gene was over-expressed, and miRNA-21 expression were measured Huh 7 Hep G2 cells. over-expressed these cells, target proteins analyzed. To confirm role proliferation, G 2.2.1.5 cells (a line expresses stably) used for inhibition studies. over-expression enhanced (3.7- 4.5-fold increase; n = 3; p<0.01) (24- 36-fold increase, normalized with 5S rRNA; p<0.001) respectively. also resulted PDCD4 PTEN. a significant increase (2- 2.3-fold over control cells; p<0.05 respectively) decreased PTEN expression. Anti-miR-21 decrease (p<0.05) increased protein We conclude infection at least part, during progression.