Boric Acid Inhibits Germination and Colonization of Saprolegnia Spores In Vitro and In Vivo

• DOI: 10.1371/journal.pone.0091878
• Publication Date: 2014-04-03T20:37:21Z
• Authors: Shimaa E. Ali; Even Thoen; Øystein Evensen; Ida Skaar
• Keywords: Saprolegnia; Boric acid; Spore germination

Saprolegnia infections cause severe economic losses among freshwater fish and their eggs. The banning of malachite green increased the demand for finding effective alternative treatments to control disease. In present study, we investigated ability boric acid saprolegniosis in salmon eggs yolk sac fry. Under vitro conditions, was able decrease spore activity mycelial growth all tested concentrations above 0.2 g/L, while complete inhibition germination observed at a concentration 0.8 g/L. vivo experiments using Atlantic eyed eggs, controlled by ranging from 0.2–1.4 g/L during continuous exposure, 1.0–4.0 intermittent exposure. same effect on fry exposed continuously 0.5 natural outbreak saprolegniosis. During no negative impact with regard hatchability viability either or fry, which indicate safety use concentrations. high survival rates recorded following testing suggest that is candidate prophylaxis