Age-Related Decrease of Meiotic Cohesins in Human Oocytes

• DOI: 10.1371/journal.pone.0096710
• Publication Date: 2014-05-07T20:29:31Z
• Authors: Makiko Tsutsumi; Reiko Fujiwara; Haruki Nishizawa; Mayuko Ito; Hiroshi Kogo; Hidehito Inagaki; Tamae Ohye; Takema Kato; Takuma Fujii; Hiroki Kurahashi
• Keywords: Meiosis II

Aneuploidy in fetal chromosomes is one of the causes pregnancy loss and congenital birth defects. It known that frequency oocyte aneuploidy increases with human maternal age. Recent data have highlighted contribution cohesin complexes correct segregation meiotic chromosomes. In mammalian oocytes, cohesion established during stages meiosis-specific subunits are not replenished after birth, raising possibility long arrest oocytes facilitates a deterioration leads to age-related aneuploidy. We here examined levels dictyate from different age groups humans mice by immunofluorescence analyses ovarian sections. The subunits, REC8 SMC1B, were found be decreased women aged 40 over compared those around 20 years (P<0.01). Age-related decreases cohesins also evident mice. Interestingly, SMC1A, mitotic counterpart was substantially detectable but little expressed Further, amount slightly increased These results suggest that, may operate coordinated way maintain cohesions sustained period impair sister chromatid leading errors.