Aerosol Mycobacterium tuberculosis Infection Causes Rapid Loss of Diversity in Gut Microbiota

• DOI: 10.1371/journal.pone.0097048
• Publication Date: 2014-05-12T17:26:43Z
• Authors: Kathryn Winglee; Emiley Eloe-Fadrosh; Shashank Gupta; Haidan Guo; Claire Fraser; William Bishai
• Keywords: Aerosols; 0301 basic medicine; Sequence Analysis, RNA; Science; Microbiota; Q; R; Biodiversity; Mycobacterium tuberculosis; 3. Good health; Gastrointestinal Tract; Mice; 03 medical and health sciences; RNA, Ribosomal, 16S; Medicine; Animals; Female; Research Article

Mycobacterium tuberculosis is an important human pathogen, and yet diagnosis remains challenging. Little research has focused on the impact of M. gut microbiota, despite significant immunological homeostatic functions gastrointestinal tract. To determine effect infection we followed mice from aerosol until death, using 16S rRNA sequencing. We saw a rapid change in microbiota response to infection, with all showing loss then recovery microbial community diversity, found that pre-infection samples clustered separately post-infection samples, ecological beta-diversity measures. The fecal was observed as rapidly six days following lung infection. Analysis additional infected by different strain corroborated these results, together demonstrating mouse significantly changes