Inhibiting Sperm Pyruvate Dehydrogenase Complex and Its E3 Subunit, Dihydrolipoamide Dehydrogenase Affects Fertilization in Syrian Hamsters

• DOI: 10.1371/journal.pone.0097916
• Publication Date: 2014-05-22T20:50:09Z
• Authors: Archana B. Siva; Subbarayalu Panne...; Purnima Sailasree; Durgesh K. Singh; Duvurri B. Kamesh...; Sisinthy Shivaji
• Keywords: Dihydrolipoamide dehydrogenase

Background/Aims The importance of sperm capacitation for mammalian fertilization has been confirmed in the present study via metabolism. Involvement metabolic enzymes pyruvate dehydrogenase complex (PDHc) and its E3 subunit, dihydrolipoamide (DLD) hamster vitro (IVF) is being proposed through regulation intracellular lactate, pH calcium. Methodology Principal Findings Capacitated spermatozoa were allowed to fertilize oocytes which then assessed fertilization, microscopically. PDHc/DLD was inhibited by use specific DLD-inhibitor, MICA (5-methoxyindole-2-carboxylic acid). Oocytes fertilized with MICA-treated (MT) [and thus PDHc/DLD-inhibited] showed defective where 2nd polar body release pronuclei formation not observed. Defective attributable failure owing high lactate low calcium MT-spermatozoa during capacitation. Moreover, this defect could be overcome alkalinizing spermatozoa, before fertilization. Increasing pre-IVF defectively-fertilized oocytes, post-fertilization rescued arrest seen, suggesting role from either gametes Parallel experiments carried out control capacitated medium extracellular or substantiated necessity optimal levels, capacitation, proper Conclusions This confirms pyruvate/lactate metabolism capacitating successful besides revealing first time PDHc/ DLD modulation In addition, observations made IVF studies hamsters suggest that failures a plausible cause unsuccessful encountered human assisted reproductive technologies, like ICSI. Our indicate post-penetration events