Transcutaneous Carbon Dioxide Induces Mitochondrial Apoptosis and Suppresses Metastasis of Oral Squamous Cell Carcinoma In Vivo

• DOI: 10.1371/journal.pone.0100530
• Publication Date: 2014-07-02T17:30:02Z
• Authors: Daisuke Takeda; Takumi Hasegawa; Takeshi Ueha; Yusuke Imai; Akiko Sakakibara; Masaya Minoda; Teruya Kawamoto; Tsutomu Minamikawa; Yasuyuki Shibuya; Toshihiro Akisue; Yoshitada Sakai; Masahiro Kurosaka; Takahide Komori
• Keywords: TFAM

Squamous cell carcinoma (SCC) is the main histological type of oral cancer. Its growth rate and incidence metastasis to regional lymph nodes influenced by various factors, including hypoxic conditions. We have previously reported that transcutaneous CO2 induces mitochondrial apoptosis decreases lung reoxygenating sarcoma cells. However, previous studies not determined sequential mechanism which suppresses epithelial tumors, SCCs. Moreover, there no report lymphogenous using human lines xenografts. In this study, we examined effects on cancer SCC Our results showed affects expressions PGC-1α TFAM protein levels cleavage products caspase-3, caspase-9 PARP, relatives apoptosis. They also significantly inhibits tumor HIF-1α, VEGF, MMP-2 MMP-9, play essential roles in angiogenesis, invasion metastasis. conclusion, suppressed growth, increased decreased number node decreasing intra-tumoral hypoxia suppressing metastatic potential with observable effect vivo. findings indicate could be a novel therapeutic tool for treating SCC.