Visualization of Phosphatidic Acid Fluctuations in the Plasma Membrane of Living Cells

• DOI: 10.1371/journal.pone.0102526
• Publication Date: 2014-07-15T17:26:37Z
• Authors: José P. Ferraz-No...; F. Javier Díez-Gu...; Juan Llopis
• Keywords: Phospholipase D; Filopodia; Lamellipodium; Pseudopodia; Cell membrane

We developed genetically-encoded fluorescent sensors based on Förster Resonance Energy Transfer to monitor phosphatidic acid (PA) fluctuations in the plasma membrane using Spo20 as PA-binding motif. Basal PA levels and phospholipase D activity varied different cell types. In addition, stimuli that activate phosphatases, leading lower levels, increased lamellipodia filopodia formation. Lower were observed edge than trailing of migrating HeLa cells. MSC80 OLN93 cells, which are stable lines derived from Schwann cells oligodendrocytes, respectively, a higher ratio diacylglycerol was demonstrated processes involved myelination, compared body. propose reported here valuable tools unveil role variety intracellular signaling pathways.