Chikungunya Virus Exploits miR-146a to Regulate NF-κB Pathway in Human Synovial Fibroblasts

• DOI: 10.1371/journal.pone.0103624
• Publication Date: 2014-08-01T18:50:59Z
• Authors: Sakthi Priya Selv...; Ritu Mishra; Sunit K. Singh
• Keywords: Pathogenesis

Objectives Chikungunya virus causes chronic infection with manifestations of joint pain. Human synovial fibroblasts get infected CHIKV and could lead to pro-inflammatory responses. MicroRNAs have potentials regulate the gene expression various anti-viral genes. The study aims investigate role miR-146a in modulation inflammatory responses human by virus. Methods To pathogenesis cells underlying manifestations, we performed primary fibroblasts. Western blotting, real-time PCR, luciferase reporter assay, overexpression knockdown cellular strategies been employed validate regulation NF-κB pathway. Results induced miR-146a, which resulted into down-regulation TRAF6, IRAK1, IRAK2 increased replication Exogenous led decreased CHIKV. Inhibition anti-miR-146a restored levels IRAK1 IRAK2. Downregulation downstream activation through negative feedback loop. Conclusion This demonstrated mechanism exploitation modulating host antiviral immune response