Performance of Simplexa Dengue Molecular Assay Compared to Conventional and SYBR Green RT-PCR for Detection of Dengue Infection in Indonesia

• DOI: 10.1371/journal.pone.0103815
• Publication Date: 2014-08-07T18:11:43Z
• Authors: R. Tedjo Sasmono; Aryati Aryati; Puspa Wardhani; Benediktus Yohan; Hidayat Trimarsanto; Sukmal Fahri; Tri Y. Setianingsih; Febrina Meutiawati
• Keywords: SYBR Green I

Diagnostic tests based on detection of dengue virus (DENV) genome are available with varying sensitivities and specificities. The Simplexa Dengue assay (Focus Diagnostics) is a newly developed real-time RT-PCR method designed to detect serotype DENV simultaneously. To assess the performance assay, we performed comparison conventional SYBR Green patients sera isolated from eight cities across Indonesia, endemic country. A total 184 that were confirmed using NS1 and/or IgM IgG ELISA examined. Using RT-PCR, detected in 53 (28.8%) 81 (44.0%) out sera, respectively. When was employed, rate increased 76.6% (141 samples). tested 40 by isolation as gold standard, yielded 95% sensitivity while reached 97.5% 100%, specificities all methods 100% when 43 non-dengue illness 20 healthy human samples. Altogether, our data showed higher compared field/surveillance setting. In conclusion, offers rapid accurate typing infection suitable for both routine diagnostic surveillance.