QTLs for Tolerance of Drought and Breeding for Tolerance of Abiotic and Biotic Stress: An Integrated Approach

• DOI: 10.1371/journal.pone.0109574
• Publication Date: 2014-10-14T18:01:01Z
• Authors: Shalabh Dixit; B. Emma Huang; Ma. Teresa Sta Cruz; Paul T. Maturan; Jhon Christian E....; Arvind Kumar
• Keywords: Epistasis; Drought Tolerance; Oryza; Marker-Assisted Selection

Background The coupling of biotic and abiotic stresses leads to high yield losses in rainfed rice (Oryza sativa L.) growing areas. While several studies target these independently, breeding strategies combat multiple seldom exist. This study reports an integrated strategy that combines QTL mapping phenotypic selection develop lines with grain (GY) under drought stress non-stress conditions, tolerance blast. Methodology A blast-tolerant BC2F3-derived population was developed from the cross tropical japonica cultivar Moroberekan (blast- drought-tolerant) high-yielding indica variety Swarna drought-susceptible) through for blast at BC2F2 generation. studied segregation distortion patterns QTLs GY were identified along epistatic interactions trait. Results Segregation distortion, favour Moroberekan, observed 50 59 loci. Majority marker loci co-localized known or NBS-LRR disease resistance genes. Despite presence variation DTF, PH conditions three traits. Epistatic also detected which explained a large proportion variance population. Conclusions allowed us identify rapid development purelines tolerant considerably reduced efforts. Apart this, it effects cycle tolerance. screened IRRI environment, identified. With two major potential, may provide stability