Effects of miR-33a-5P on ABCA1/G1-Mediated Cholesterol Efflux under Inflammatory Stress in THP-1 Macrophages

• DOI: 10.1371/journal.pone.0109722
• Publication Date: 2014-10-20T21:38:45Z
• Authors: Min Mao; Han Lei; Qing Liu; Yaxi Chen; Lei Zhao; Qing Li; Suxin Luo; Zhong Zuo; Quan He; Wei Huang; Nan Zhang; Chao Zhou; Xiong Z. Ruan
• Keywords: ABCG1; THP1 cell line; Proinflammatory cytokine; Efflux

The present study is to investigate whether inflammatory cytokines inhibit ABCA1/ABCG1-mediated cholesterol efflux by regulating miR-33a-5P in THP-1 macrophages. We used interleukin-6 and tumor necrosis factor-alpha the presence or absence of native low density lipoprotein (LDL) stimulate macrophages were infected either control lentivirus vectors encoding antisense miR-33a-5P. effects cytokines, on intracellular lipids accumulation contents assessed oil red O staining quantitative assay. ApoA-I-mediated was examined using fluorescent sterol (BODIPY-cholesterol). gene protein expressions molecules involved trafficking real-time polymerase chain reaction Western blotting. Inflammatory increased lipid decreased apoA-I-mediated via decreasing expression ABCA1 ABCG1 LDL However, reversed accumulation, efflux, miR-33a-5P, This indicated that inhibited up-regulating