Prevalence of Veterinary Antibiotics and Antibiotic-Resistant Escherichia coli in the Surface Water of a Livestock Production Region in Northern China

• DOI: 10.1371/journal.pone.0111026
• Publication Date: 2014-11-05T19:12:23Z
• Authors: Xuelian Zhang; Yanxia Li; Bei Liu; Jing Wang; Chenghong Feng; Min Gao; Lina Wang
• Keywords: China; Livestock; Science; Microbial Sensitivity Tests; 01 natural sciences; Animal Diseases; Rivers; Drug Resistance, Bacterial; Escherichia coli; Prevalence; Animals; Escherichia coli Infections; 0105 earth and related environmental sciences; 2. Zero hunger; Q; R; Agriculture; 15. Life on land; 6. Clean water; Anti-Bacterial Agents; 3. Good health; Medicine; Water Microbiology; Water Pollutants, Chemical; Research Article; Environmental Monitoring

This study investigated the occurrence of 12 veterinary antibiotics (VAs) and the susceptibility of Escherichia coli (E. coli) in a rural water system that was affected by livestock production in northern China. Each of the surveyed sites was determined with at least eight antibiotics with maximum concentration of up to 450 ng L(-1). The use of VAs in livestock farming probably was a primary source of antibiotics in the rivers. Increasing total antibiotics were measured from up- to mid- and downstream in the two tributaries. Eighty-eight percent of the 218 E. coli isolates that were derived from the study area exhibited, in total, 48 resistance profiles against the eight examined drugs. Significant correlations were found among the resistance rates of sulfamethoxazole-trimethoprim, chloromycetin and ampicillin as well as between tetracycline and chlortetracycline, suggesting a possible cross-selection for resistance among these drugs. The E. coli resistance frequency also increased from up- to midstream in the three rivers. E. coli isolates from different water systems showed varying drug numbers of resistance. No clear relationship was observed in the antibiotic resistance frequency with corresponding antibiotic concentration, indicating that the antibiotic resistance for E. coli in the aquatic environment might be affected by factors besides antibiotics. High numbers of resistant E. coli were also isolated from the conserved reservoir. These results suggest that rural surface water may become a large pool of VAs and resistant bacteria. This study contributes to current information on VAs and resistant bacteria contamination in aquatic environments particularly in areas under intensive agriculture. Moreover, this study indicates an urgent need to monitor the use of VAs in animal production, and to control the release of animal-originated antibiotics into the environment.