Regulation of Coagulation Factor XI Expression by MicroRNAs in the Human Liver

• DOI: 10.1371/journal.pone.0111713
• Publication Date: 2014-11-07T18:53:49Z
• Authors: Salam Salloum-Asfar; Raúl Teruel-Montoya; Ana B. Arroyo; Nuria García-Barberá; Amarjit Chaudhry; Erin Schuetz; Ginés Luengo-Gil; Vicente Vicente; Rocío González-Co...; Constantino Martínez
• Keywords: 0301 basic medicine; Base Sequence; Science; Q; R; MicroRNAs; 03 medical and health sciences; Gene Expression Regulation; Liver; Medicine; Humans; RNA, Messenger; Factor XI; Research Article; Oligonucleotide Array Sequence Analysis

High levels of factor XI (FXI) increase the risk thromboembolic disease. However, genetic and environmental factors regulating FXI expression are still largely unknown. The aim our study was to evaluate regulation by microRNAs (miRNAs) in human liver. In silico prediction yielded four miRNA candidates that might regulate expression. HepG2 cells were transfected with miR-181a-5p, miR-23a-3p, miR-16-5p miR-195-5p. We used mir-494, which not predicted bind F11, as a negative control. Only miR-181a-5p caused significant decrease both protein F11 mRNA levels. addition, transfection inhibitor PLC/PRF/5 hepatic increased extracellular FXI. Luciferase assays colon cancer deficient for Dicer (HCT-DK) demonstrated direct interaction between 3′untranslated region F11. Additionally, inversely significantly correlated 114 healthy livers, but miR-494. This demonstrates is directly regulated specific miRNA, Future studies necessary further investigate potential consequences dysregulation pathologies involving