Combination of Silver Nanoparticles and Drosera binata Extract as a Possible Alternative for Antibiotic Treatment of Burn Wound Infections Caused by Resistant Staphylococcus aureus

• DOI: 10.1371/journal.pone.0115727
• Publication Date: 2014-12-31T18:44:04Z
• Authors: Marta Krychowiak; Mariusz Grinholc; Rafal Banasiuk; Miroslawa Krauze-...; Daniel Głód; Anna Kawiak; Aleksandra Królicka
• Keywords: Pathogenic bacteria; Silver nanoparticle

Staphylococcus aureus is the most common infectious agent involved in development of skin infections that are associated with antibiotic resistance, such as burn wounds. As drug resistance a growing problem it essential to establish novel antimicrobials. Currently, bacteria successfully controlled by multi-drug therapies. Here we demonstrate secondary metabolites present extract obtained from Drosera binata vitro cultures effective antibacterial agents against S. grown planktonic culture and biofilm. Moreover, this first report demonstrating synergistic interaction between D. silver nanoparticles (AgNPs), which results spectacular enhancement observed bactericidal activity, while having no cytotoxic effects on human keratinocytes. Simultaneous use these two significantly reduced quantities produces same effect, i.e. killing 99.9% inoculum or eradicating staphylococcal biofilm, higher amounts used individually. Our data indicates combining AgNPs either its pure compound (3-chloroplumbagin) may provide safe highly alternative commonly antibiotics, ineffective towards antibiotic-resistant aureus.