Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response

• DOI: 10.1371/journal.pone.0116697
• Publication Date: 2015-02-06T21:44:45Z
• Authors: Dezhou Cui; Dandan Wu; Jie Liu; Detao Li; Chunyan Xu; Song Li; Peng Li; Hua Zhang; Xu Liu; Chuan Jiang; Liwen Wang; Tingting Chen; Huabang Chen; Li Zhao
• Keywords: 0301 basic medicine; genotype; Arabidopsis; maize; Plant Roots; 630; cysteine proteinase; adenosylhomocysteinase; histone H4; plant structures; plant protein; pathogenesis related protein 10; adenine nucleotide translocase; vegetable protein; sucrose synthase; comparative study; Plant Proteins; 2. Zero hunger; potassium; Q; seed maturation protein PM41; R; plasma membrane intrinsic protein 2; plasma membrane intrinsic protein 1; sodium ion; gene control; Salt Tolerance; exhydrolase II isoform 1; cross tolerance; unclassified drug; genotype F35; sodium chloride; Medicine; down regulation; Research Article; 570; Genotype; lichenase 2 precursor; phenotype; Science; water; formate dehydrogenase; ribosomal protein S8; Zea mays; Article; osmotic potential; 03 medical and health sciences; alpha 1,4 glucan protein synthase; Species Specificity; Stress, Physiological; controlled study; heat shock protein 90; genotype F63; protein expression; maize insect resistance 1; nonhuman; plant root; peroxidase 42 precursor; protein GST 4; barley; aquaporin; fructose bisphosphate aldolase; xyloglucan endotransglycosylase; protein 14 3 3; plasma membrane intrinsic protein[2-4]; inbred strain; fructokinase 2

Salinity is a major abiotic stress that limits plant productivity and quality throughout the world. Roots are the sites of salt uptake. To better understand salt stress responses in maize, we performed a comparative proteomic analysis of seedling roots from the salt-tolerant genotype F63 and the salt-sensitive genotype F35 under 160 mM NaCl treatment for 2 days. Under salinity conditions, the shoot fresh weight and relative water content were significantly higher in F63 than in F35, while the osmotic potential was significantly lower and the reduction of the K+/Na+ ratio was significantly less pronounced in F63 than in F35. Using an iTRAQ approach, twenty-eight proteins showed more than 2.0- fold changes in abundance and were regarded as salt-responsive proteins. Among them, twenty-two were specifically regulated in F63 but remained constant in F35. These proteins were mainly involved in signal processing, water conservation, protein synthesis and biotic cross-tolerance, and could be the major contributors to the tolerant genotype of F63. Functional analysis of a salt-responsive protein was performed in yeast as a case study to confirm the salt-related functions of detected proteins. Taken together, the results of this study may be helpful for further elucidating salt tolerance mechanisms in maize.