Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress

• DOI: 10.1371/journal.pone.0117331
• Publication Date: 2015-02-23T19:20:58Z
• Authors: Ricardo Jasso-Chávez; M. Geovanni Santi...; Elizabeth Lira-Silva; Erika Pineda; Armando Zepeda-Ro...; Javier Belmont-Díaz; Rusely Encalada; Emma Saavedra; Rafael Moreno-Sán...
• Keywords: 2. Zero hunger; 0301 basic medicine; Science; Air; Q; R; Oxygen; Genome, Microbial; Oxidative Stress; 03 medical and health sciences; Polyphosphates; Methanosarcina; Medicine; Reactive Oxygen Species; Methane; Research Article; Peroxidase

Methanosarcina acetivorans, considered a strict anaerobic archaeon, was cultured in the presence of 0.4–1% O2 (atmospheric) for at least 6 months to generate air-adapted cells; further, biochemical mechanisms developed deal with were characterized. Methane production and protein content, as indicators cell growth, did not change cells respect under anoxia (control cells). In contrast, growth methane significantly decreased control exposed first time O2. Production reactive oxygen species 50 times lower versus cells, suggesting enhanced anti-oxidant that attenuated toxicity. this regard, (i) transcripts activities superoxide dismutase, catalase peroxidase increased; (ii) thiol-molecules (cysteine + coenzyme M-SH sulfide) polyphosphate contents respectively 2 5 higher anaerobic-control cells. Long-term cultures (18 days) 2% exhibited ability form biofilms. These data indicate M. acetivorans develops multiple contend associated oxidative stress, also suggested by genome analyses some methanogens.