Genetic Diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and Identification of a Rare Multidrug Resistant Beijing Genotype

• DOI: 10.1371/journal.pone.0118095
• Publication Date: 2015-02-19T18:47:36Z
• Authors: Samantha Flores-T...; Rayo Morfín-Otero; Eduardo Rodríguez...; Esteban González-...; Héctor R. Pérez-G...; Virgilio Bocanegr...; Lucio Vera-Cabrera; Elvira Garza-Gonz...
• Keywords: Adult; DNA, Bacterial; Male; 0301 basic medicine; Adolescent; Genotype; Science; 610; Microbial Sensitivity Tests; 03 medical and health sciences; Drug Resistance, Multiple, Bacterial; 616; Cluster Analysis; Humans; Child; Mexico; Aged; Aged, 80 and over; Q; R; Genetic Variation; Infant; Middle Aged; Bacterial Typing Techniques; 3. Good health; Beijing; Child, Preschool; Medicine; Female; Research Article

Determining the genetic diversity of M. tuberculosis strains allows identification distinct Mycobacterium genotypes responsible for in different regions. Several studies have reported Mexico, but little information is available from state Jalisco. Therefore, aim this study was to determine clinical isolates Western Mexico. Sixty-eight were tested susceptibility first-line drugs using manual Mycobacteria Growth Indicator Tube method and genotyped spoligotyping IS6110-restriction fragment length polymorphism (RFLP) pattern analyses. Forty-seven (69.1%) grouped into 10 clusters 21 displayed single patterns by spoligotyping. Three corresponded orphan SITVITWEB database, 1 new type that contained 2 created. The most prevalent lineages T (38.2%), Haarlem (17.7%), LAM X (7.4%), S (5.9%), EAI (1.5%) Beijing (1.5%). Six (12.8%) clustered MDR, 406 family among MDR isolates. Seventeen (26.2%) 8 48 IS6110-RFLP. Combination IS6110-RFLP reduced clustering rate 20.0%. results show T, Haarlem, are predominant Guadalajara, Clustering rates indicated low transmission strains. We detected a rare genotype, SIT406, which highly resistant strain. This first report genotype Latin America.